Project Experience
Project Case
FAQ
1. Cell source: CHO cells are a cell lines derived from the Chinese hamster ovary, while 293 cells are transformed from human embryonic kidney (HEK) cells.
2. Growth characteristics: CHO cells grow rapidly, adapt to a variety of culture conditions, and are easy to expand; while 293 cells have a slower growth rate and require higher culture temperatures and CO2 concentration.
3. Expression ability: CHO cells are widely used in the eukaryotic protein expression, especially in expressing complex proteins such as antibodies, while 293 cells are widely used in the expression of recombinant proteins, viral vectors and other fields.
1. High GC content: GC base pairs have strong bonding, which easily leads to stable secondary structures that interfere with transcription and translation.
2. Abnormal promoters and regulatory sequences.
3. Structural domains and translational slow-down regions.
4. Sequences that are difficult to fold stably.
5. Sequences with extensive glycosylation or high cysteine content.
1. Culture scale: Large-scale antibody expression typically requires large-scale culturing using larger flasks, bioreactors or fermenters. In contrast, small-scale expression can be performed in smaller vessels such as culture dishes or centrifuge tubes.
2. Cell density control: Large-scale expression requires maintaining high cell density for increased yield which involves optimizing nutrient supply and media compositions. Small-scale setups can operate at lower cell densities, with more focus on protein purification and downstream analysis;
3. Expression optimization: Large-scale production emphasizes optimizing induction conditions, transfection efficiency and culture parameters to maximize yield and purity. In contrast, small-scale expression focuses on maintaining stable expression levels and minimizing cytotoxicity.
4. Protein purification: Large-scale expression generates more protein and typically involves multi-step purification. Small-scale expression often uses simpler techniques such as affinity chromatography or gel filtration.
1. Use protease-deficient CHO cells or addition of protease inhibitors (e.g. leupastatin).
2. Shorten culture duration and store products at low temperatures (<15℃).
3. Adjust pH (6.8-7.2) to inhibit protease activity.
1. Cell source: CHO cells are a cell lines derived from the Chinese hamster ovary, while 293 cells are transformed from human embryonic kidney (HEK) cells.
2. Growth characteristics: CHO cells grow rapidly, adapt to a variety of culture conditions, and are easy to expand; while 293 cells have a slower growth rate and require higher culture temperatures and CO2 concentration.
3. Expression ability: CHO cells are widely used in the eukaryotic protein expression, especially in expressing complex proteins such as antibodies, while 293 cells are widely used in the expression of recombinant proteins, viral vectors and other fields.
1. High GC content: GC base pairs have strong bonding, which easily leads to stable secondary structures that interfere with transcription and translation.
2. Abnormal promoters and regulatory sequences.
3. Structural domains and translational slow-down regions.
4. Sequences that are difficult to fold stably.
5. Sequences with extensive glycosylation or high cysteine content.
1. Culture scale: Large-scale antibody expression typically requires large-scale culturing using larger flasks, bioreactors or fermenters. In contrast, small-scale expression can be performed in smaller vessels such as culture dishes or centrifuge tubes.
2. Cell density control: Large-scale expression requires maintaining high cell density for increased yield which involves optimizing nutrient supply and media compositions. Small-scale setups can operate at lower cell densities, with more focus on protein purification and downstream analysis;
3. Expression optimization: Large-scale production emphasizes optimizing induction conditions, transfection efficiency and culture parameters to maximize yield and purity. In contrast, small-scale expression focuses on maintaining stable expression levels and minimizing cytotoxicity.
4. Protein purification: Large-scale expression generates more protein and typically involves multi-step purification. Small-scale expression often uses simpler techniques such as affinity chromatography or gel filtration.
1. Use protease-deficient CHO cells or addition of protease inhibitors (e.g. leupastatin).
2. Shorten culture duration and store products at low temperatures (<15℃).
3. Adjust pH (6.8-7.2) to inhibit protease activity.
Project Experience
Project Case
FAQ
1. Cell source: CHO cells are a cell lines derived from the Chinese hamster ovary, while 293 cells are transformed from human embryonic kidney (HEK) cells.
2. Growth characteristics: CHO cells grow rapidly, adapt to a variety of culture conditions, and are easy to expand; while 293 cells have a slower growth rate and require higher culture temperatures and CO2 concentration.
3. Expression ability: CHO cells are widely used in the eukaryotic protein expression, especially in expressing complex proteins such as antibodies, while 293 cells are widely used in the expression of recombinant proteins, viral vectors and other fields.
1. High GC content: GC base pairs have strong bonding, which easily leads to stable secondary structures that interfere with transcription and translation.
2. Abnormal promoters and regulatory sequences.
3. Structural domains and translational slow-down regions.
4. Sequences that are difficult to fold stably.
5. Sequences with extensive glycosylation or high cysteine content.
1. Culture scale: Large-scale antibody expression typically requires large-scale culturing using larger flasks, bioreactors or fermenters. In contrast, small-scale expression can be performed in smaller vessels such as culture dishes or centrifuge tubes.
2. Cell density control: Large-scale expression requires maintaining high cell density for increased yield which involves optimizing nutrient supply and media compositions. Small-scale setups can operate at lower cell densities, with more focus on protein purification and downstream analysis;
3. Expression optimization: Large-scale production emphasizes optimizing induction conditions, transfection efficiency and culture parameters to maximize yield and purity. In contrast, small-scale expression focuses on maintaining stable expression levels and minimizing cytotoxicity.
4. Protein purification: Large-scale expression generates more protein and typically involves multi-step purification. Small-scale expression often uses simpler techniques such as affinity chromatography or gel filtration.
1. Use protease-deficient CHO cells or addition of protease inhibitors (e.g. leupastatin).
2. Shorten culture duration and store products at low temperatures (<15℃).
3. Adjust pH (6.8-7.2) to inhibit protease activity.
1. Cell source: CHO cells are a cell lines derived from the Chinese hamster ovary, while 293 cells are transformed from human embryonic kidney (HEK) cells.
2. Growth characteristics: CHO cells grow rapidly, adapt to a variety of culture conditions, and are easy to expand; while 293 cells have a slower growth rate and require higher culture temperatures and CO2 concentration.
3. Expression ability: CHO cells are widely used in the eukaryotic protein expression, especially in expressing complex proteins such as antibodies, while 293 cells are widely used in the expression of recombinant proteins, viral vectors and other fields.
1. High GC content: GC base pairs have strong bonding, which easily leads to stable secondary structures that interfere with transcription and translation.
2. Abnormal promoters and regulatory sequences.
3. Structural domains and translational slow-down regions.
4. Sequences that are difficult to fold stably.
5. Sequences with extensive glycosylation or high cysteine content.
1. Culture scale: Large-scale antibody expression typically requires large-scale culturing using larger flasks, bioreactors or fermenters. In contrast, small-scale expression can be performed in smaller vessels such as culture dishes or centrifuge tubes.
2. Cell density control: Large-scale expression requires maintaining high cell density for increased yield which involves optimizing nutrient supply and media compositions. Small-scale setups can operate at lower cell densities, with more focus on protein purification and downstream analysis;
3. Expression optimization: Large-scale production emphasizes optimizing induction conditions, transfection efficiency and culture parameters to maximize yield and purity. In contrast, small-scale expression focuses on maintaining stable expression levels and minimizing cytotoxicity.
4. Protein purification: Large-scale expression generates more protein and typically involves multi-step purification. Small-scale expression often uses simpler techniques such as affinity chromatography or gel filtration.
1. Use protease-deficient CHO cells or addition of protease inhibitors (e.g. leupastatin).
2. Shorten culture duration and store products at low temperatures (<15℃).
3. Adjust pH (6.8-7.2) to inhibit protease activity.
+86 21 3368 1627
protein@sanyoubio.com
abx.sanyoubio.com.cn
